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Table S1. Observation of aggregates (Agg.) and flakes in all formulations except of PHB-NP prepared by water and Tween 80 (1% v/v) as polar phase and surfactant, respectively.

Figure S1. Morphology of PHB fibers, flake, and particles (A) and size distribution of colloidal part of suspension (B) prepared by nanoprecipitation method with Tween 80 (0.1% v/v).

Figure S2. TGA and DSC thermograms of unloaded PHB nanoparticles.

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