C: Food Chemistry
A Fast and Validated Method for the Determination of Malondialdehyde in Fish Liver Using High-Performance Liquid Chromatography with a Photodiode Array Detector
Article first published online: 12 MAR 2014
© 2014 Institute of Food Technologists®
Journal of Food Science
Volume 79, Issue 4, pages C484–C488, April 2014
How to Cite
Faizan, M., Esatbeyoglu, T., Bayram, B. and Rimbach, G. (2014), A Fast and Validated Method for the Determination of Malondialdehyde in Fish Liver Using High-Performance Liquid Chromatography with a Photodiode Array Detector. Journal of Food Science, 79: C484–C488. doi: 10.1111/1750-3841.12412
- Issue published online: 8 APR 2014
- Article first published online: 12 MAR 2014
- Manuscript Accepted: 23 JAN 2014
- Manuscript Received: 4 SEP 2013
- lipid peroxidation;
- polyunsaturated fatty acids
Malondialdehyde (MDA) is a biomarker of lipid peroxidation and is present in foods and biological samples such as plasma. A high-performance liquid chromatography (HPLC) method was applied to determine MDA in fish liver samples after derivatization with 2,4-dinitrophenylhydrazine (DNPH) using a ODS2 column (10 cm × 4.6 mm, 3 μm) and a photodiode array detector. The mobile phase consisted of 0.2% acetic acid (v/v) in distilled water and acetonitrile (42:58, v/v). The present method was validated in terms of linearity, lower limit of quantification, lower limit of detection, precision, accuracy, recovery, and stability of MDA according to U.S. Food and Drug Administration (FDA) guidelines. The limit of quantification of MDA was 0.39 μmol/L, which is comparable to other methods. The recovery of the spiked MDA liver samples was in the range of 92.4% to 104.2%. This newly modified HPLC method is specific, sensitive, and accurate and allows the analysis of MDA within 4 min in fish liver but also in other tissues and plasma.
Malondialdehyde is an established biomarker of lipid peroxidation in foods. We developed a fast, simple, and sensitive HPLC method with photodiode array detection using DNPH for derivatization of malondialdehyde in fish liver.