Funding Information The work was supported by the project GACR No. P503/10/0408, Grant No. TA01020804 of the Czech Technology Agency and by the Institutional Research Concept RVO:61388971.
Transcriptional response of lignin-degrading enzymes to 17α-ethinyloestradiol in two white rots
Article first published online: 22 NOV 2012
© 2013 The Authors. Microbial Biotechnology published by Blackwell Publishing Ltd and Society for Applied Microbiology
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Special Issue: Plant-Microbe Interactions
Volume 6, Issue 3, pages 300–306, May 2013
How to Cite
Přenosilová, L., Křesinová, Z., Amemori, A. S., Cajthaml, T. and Svobodová, K. (2013), Transcriptional response of lignin-degrading enzymes to 17α-ethinyloestradiol in two white rots. Microbial Biotechnology, 6: 300–306. doi: 10.1111/1751-7915.12007
- Issue published online: 15 APR 2013
- Article first published online: 22 NOV 2012
- Manuscript Accepted: 6 OCT 2012
- Manuscript Revised: 4 OCT 2012
- Manuscript Received: 30 JUL 2012
- Czech Technology Agency. Grant Number: TA01020804
- Institutional Research Concept RVO. Grant Number: 61388971
Fungal, ligninolytic enzymes have attracted a great attention for their bioremediation capabilities. A deficient knowledge of regulation of enzyme production, however, hinders the use of ligninolytic fungi in bioremediation applications. In this work, a transcriptional analyses of laccase and manganese peroxidase (MnP) production by two white rots was combined with determination of pI of the enzymes and the evaluation of 17α-ethinyloestradiol (EE2) degradation to study regulation mechanisms used by fungi during EE2 degradation. In the cultures of Trametes versicolor the addition of EE2 caused an increase in laccase activity with a maximum of 34.2 ± 6.7 U g−1 of dry mycelia that was observed after 2 days of cultivation. It corresponded to a 4.9 times higher transcription levels of a laccase-encoding gene (lacB) that were detected in the cultures at the same time. Simultaneously, pI values of the fungal laccases were altered in response to the EE2 treatment. Like T. versicolor, Irpex lacteus was also able to remove 10 mg l−1 EE2 within 3 days of cultivation. While an increase to I. lacteus MnP activity and MnP gene transcription levels was observed at the later phase of the cultivation. It suggests another metabolic role of MnP but EE2 degradation.