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Transcriptional response of lignin-degrading enzymes to 17α-ethinyloestradiol in two white rots

Authors

  • L. Přenosilová,

    1. Laboratory of Environmental Biotechnology, Institute of Microbiology ASCR,v.v.i., Prague, Czech Republic
    2. Department of Biochemistry, Faculty of Science, Charles University in Prague, Prague 2, Czech Republic
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  • Z. Křesinová,

    1. Laboratory of Environmental Biotechnology, Institute of Microbiology ASCR,v.v.i., Prague, Czech Republic
    2. Institute of Environmental Studies, Faculty of Science, Charles University, Prague 2, Czech Republic
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  • A. Slavíková Amemori,

    1. Laboratory of Environmental Biotechnology, Institute of Microbiology ASCR,v.v.i., Prague, Czech Republic
    2. Institute of Environmental Studies, Faculty of Science, Charles University, Prague 2, Czech Republic
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  • T. Cajthaml,

    1. Laboratory of Environmental Biotechnology, Institute of Microbiology ASCR,v.v.i., Prague, Czech Republic
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  • K. Svobodová

    Corresponding author
    • Laboratory of Environmental Biotechnology, Institute of Microbiology ASCR,v.v.i., Prague, Czech Republic
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  • Funding Information The work was supported by the project GACR No. P503/10/0408, Grant No. TA01020804 of the Czech Technology Agency and by the Institutional Research Concept RVO:61388971.

For correspondence. E-mail ksvobod@biomed.cas.cz; Tel. (+420) 296442464; Fax (+420) 296442384.

Summary

Fungal, ligninolytic enzymes have attracted a great attention for their bioremediation capabilities. A deficient knowledge of regulation of enzyme production, however, hinders the use of ligninolytic fungi in bioremediation applications. In this work, a transcriptional analyses of laccase and manganese peroxidase (MnP) production by two white rots was combined with determination of pI of the enzymes and the evaluation of 17α-ethinyloestradiol (EE2) degradation to study regulation mechanisms used by fungi during EE2 degradation. In the cultures of Trametes versicolor the addition of EE2 caused an increase in laccase activity with a maximum of 34.2 ± 6.7 U g−1 of dry mycelia that was observed after 2 days of cultivation. It corresponded to a 4.9 times higher transcription levels of a laccase-encoding gene (lacB) that were detected in the cultures at the same time. Simultaneously, pI values of the fungal laccases were altered in response to the EE2 treatment. Like T. versicolor, Irpex lacteus was also able to remove 10 mg l−1 EE2 within 3 days of cultivation. While an increase to I. lacteus MnP activity and MnP gene transcription levels was observed at the later phase of the cultivation. It suggests another metabolic role of MnP but EE2 degradation.

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