Funding Information This work was supported, in part, by an ARF grant from Ministry of Education of Singapore (WBS No. R-143-000-507-112).
Yeast ratio is a critical factor for sequential fermentation of papaya wine by Williopsis saturnus and Saccharomyces cerevisiae
Article first published online: 22 NOV 2012
© 2012 The Authors. Microbial Biotechnology © 2012 Society for Applied Microbiology and John Wiley & Sons Ltd
Thematic Issue: Ecological Engineering of the Intestinal Microbiome Connecting the Environment and Food to Therapy and Health
Volume 6, Issue 4, pages 385–393, July 2013
How to Cite
Lee, P.-R., Kho, S. H. C., Yu, B., Curran, P. and Liu, S.-Q. (2013), Yeast ratio is a critical factor for sequential fermentation of papaya wine by Williopsis saturnus and Saccharomyces cerevisiae. Microbial Biotechnology, 6: 385–393. doi: 10.1111/1751-7915.12008
- Issue published online: 11 JUN 2013
- Article first published online: 22 NOV 2012
- Manuscript Accepted: 8 OCT 2012
- Manuscript Received: 28 JUL 2012
- Ministry of Education of Singapore
The growth kinetics and fermentation performance of Williopsis saturnus and Saccharomyces cerevisiae at ratios of 10:1, 1:1 and 1:10 (W.:S.) were studied in papaya juice with initial 7-day fermentation by W. saturnus, followed by S. cerevisiae. The growth kinetics of W. saturnus were similar at all ratios, but its maximum cell count decreased as the proportion of S. cerevisiae was increased. Conversely, there was an early death of S. cerevisiae at the ratio of 10:1. Williopsis saturnus was the dominant yeast at 10:1 ratio that produced papaya wine with elevated concentrations of acetate esters. On the other hand, 1:1 and 1:10 ratios allowed the coexistence of both yeasts which enabled the flavour-enhancing potential of W. saturnus as well as the ethyl ester and alcohol-producing abilities of S. cerevisiae. In particular, 1:1 and 1:10 ratios resulted in production of more ethyl esters, alcohols and 2-phenylethyl acetate. However, the persistence of both yeasts at 1:1 and 1:10 ratios led to formation of high levels of acetic acid. The findings suggest that yeast ratio is a critical factor for sequential fermentation of papaya wine by W. saturnus and S. cerevisiae as a strategy to modulate papaya wine flavour.