Funding Information This work was supported by Grant P302/12/P633 from the Czech Science Foundation, by Institutional Research Project RVO61388971 and by Grant Ka1722/1-1 from Deutsche Forschungsgemeinschaft (DFG).
Corynebacterium glutamicum promoters: a practical approach
Article first published online: 10 JAN 2013
© 2013 The Authors. Microbial Biotechnology published by Blackwell Publishing Ltd and Society for Applied Microbiology
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Special Issue: The Corynebacterium Cell Factory
Volume 6, Issue 2, pages 103–117, March 2013
How to Cite
Pátek, M., Holátko, J., Busche, T., Kalinowski, J. and Nešvera, J. (2013), Corynebacterium glutamicum promoters: a practical approach. Microbial Biotechnology, 6: 103–117. doi: 10.1111/1751-7915.12019
- Issue published online: 15 FEB 2013
- Article first published online: 10 JAN 2013
- Manuscript Accepted: 8 NOV 2012
- Manuscript Revised: 5 NOV 2012
- Manuscript Received: 14 SEP 2012
- Czech Science Foundation, by Institutional Research Project RVO61388971. Grant Number: P302/12/P633
- Deutsche Forschungsgemeinschaft (DFG). Grant Number: Ka1722/1-1
Transcription initiation is the key step in gene expression in bacteria, and it is therefore studied for both theoretical and practical reasons. Promoters, the traffic lights of transcription initiation, are used as construction elements in biotechnological efforts to coordinate ‘green waves’ in the metabolic pathways leading to the desired metabolites. Detailed analyses of Corynebacterium glutamicum promoters have already provided large amounts of data on their structures, regulatory mechanisms and practical capabilities in metabolic engineering. In this minireview the main aspects of promoter studies, the methods developed for their analysis and their practical use in C. glutamicum are discussed. These include definitions of the consensus sequences of the distinct promoter classes, promoter localization and characterization, activity measurements, the functions of transcriptional regulators and examples of practical uses of constitutive, inducible and modified promoters in biotechnology. The implications of the introduction of novel techniques, such as in vitro transcription and RNA sequencing, to C. glutamicum promoter studies are outlined.