Funding Information The authors are grateful to the financial support from a research grant from the Flemish Fund for Scientific Research (FWO-Vlaanderen, 3G070010), by the Geconcerteerde Onderzoeksactie (GOA) of Ghent University (BOF09/GOA/005) and by the Ghent University Multidisciplinary Research Partnership (MRP) – Biotechnology for a sustainable economy (01 MRA 510W).
Repeated pulse feeding induces functional stability in anaerobic digestion
Article first published online: 10 JAN 2013
© 2013 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Thematic Issue: Ecological Engineering of the Intestinal Microbiome Connecting the Environment and Food to Therapy and Health
Volume 6, Issue 4, pages 414–424, July 2013
How to Cite
De Vrieze, J., Verstraete, W. and Boon, N. (2013), Repeated pulse feeding induces functional stability in anaerobic digestion. Microbial Biotechnology, 6: 414–424. doi: 10.1111/1751-7915.12025
- Issue published online: 11 JUN 2013
- Article first published online: 10 JAN 2013
- Manuscript Accepted: 30 NOV 2012
- Manuscript Received: 7 JUL 2012
- Flemish Fund for Scientific Research. Grant Number: 3G070010
- Geconcerteerde Onderzoeksactie (GOA) of Ghent University. Grant Number: BOF09/GOA/005
- Ghent University Multidisciplinary Research Partnership (MRP) – Biotechnology for a sustainable economy. Grant Number: 01 MRA 510W
Anaerobic digestion is an environmental key technology in the future bio-based economy. To achieve functional stability, a minimal microbial community diversity is required. This microbial community should also have a certain ‘elasticity’, i.e. the ability to rapidly adapt to suboptimal conditions or stress. In this study it was evaluated whether a higher degree of functional stability could be achieved by changing the feeding pattern, which can change the evenness, dynamics and richness of the bacterial community. The first reactor (CSTRstable) was fed on daily basis, whereas the second reactor (CSTRdynamic) was fed every 2 days. Average biogas production was 0.30 l CH4 l−1 day−1 in both reactors, although daily variation was up to four times higher in the CSTRdynamic compared with the CSTRstable during the first 50 days. Bacterial analysis revealed that this CSTRdynamic had a two times higher degree of bacterial community dynamics. The CSTRdynamic also appeared to be more tolerant to an organic shock load of 8 g COD l−1 and ammonium levels up to 8000 mg TAN l−1. These results suggest that the regular application of a limited pulse of organic material and/or a variation in the substrate composition might promote higher functional stability in anaerobic digestion.