Funding Information This research was supported in part by the Samaria and Jordan Rift Valley Regional R and D Center, the Research Authority of the Ariel University Center and the Rappaport Foundation for Medical Microbiology, Bar-Ilan University (to Y.N.).
Current production in a microbial fuel cell using a pure culture of Cupriavidus basilensis growing in acetate or phenol as a carbon source
Article first published online: 10 JAN 2013
© 2013 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Thematic Issue: Ecological Engineering of the Intestinal Microbiome Connecting the Environment and Food to Therapy and Health
Volume 6, Issue 4, pages 425–434, July 2013
How to Cite
Friman, H., Schechter, A., Ioffe, Y., Nitzan, Y. and Cahan, R. (2013), Current production in a microbial fuel cell using a pure culture of Cupriavidus basilensis growing in acetate or phenol as a carbon source. Microbial Biotechnology, 6: 425–434. doi: 10.1111/1751-7915.12026
- Issue published online: 11 JUN 2013
- Article first published online: 10 JAN 2013
- Manuscript Accepted: 30 NOV 2012
- Manuscript Revised: 27 NOV 2012
- Manuscript Received: 27 JUL 2012
- Samaria and Jordan Rift Valley Regional R and D Center
- Research Authority of the Ariel University Center
- Rappaport Foundation for Medical Microbiology, Bar-Ilan University
A microbial fuel cell (MFC) was operated with a pure culture of Cupriavidus basilensis bacterial cells growing in the anode compartment in a defined medium containing acetate or phenol. Operating this mediator-less MFC under a constant external resistor of 1 kΩ with acetate or phenol led to current generation of 902 and 310 mA m−2 respectively. In the MFC which was operated using acetate or phenol, the current density measured from the plankton bacterial cells with a fresh electrode was 125 and 109 mA m−2, respectively, whereas the current obtained with biofilm-covered electrodes in sterile medium was 541 and 228 mA m−2 respectively. After 72 h in the MFC, 86% of the initial phenol concentration was removed, while only 64% was removed after the same time in the control MFC which was held at an open circuit potential (OCP). Furthermore, SEM and confocal microscopy analyses demonstrated a developed biofilm with a live C. basilensis population. In conclusion, in this study we demonstrated, for the first time, use of C. basilensis facultative aerobe bacterial cells in a MFC using acetate or phenol as the sole carbon source which led to electricity generation.