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mbt12031-sup-0001-si.docx4618K

Mathematical model for ArsR circuits.

Table S1. List of all the primers used in the present work showing sequence, length and melting temperature (Tm).

Fig. S1. Nucleotide alignment of the arsRR73 and the chromosomal arsRK12 genes.

Fig. S2. Relevant part of the DNA sequence of the different promoters used for uncoupled expression of arsRR773.

Fig. S3. Relevant construction details of the feedback (A) and uncoupled (B) circuits. Sequences show part of the arsR gene, the various promoters, the ArsR Binding Sites (ABS) and the start of the egfp reporter gene.

Fig. S4. Arsenite-dependent EGFP fluorescence in cultures of E. coli MG1655 with different uncoupled arsR reporter circuits (pAAUN, pLtetOUN, pJJUN, pVUN, pKUN) compared with the feedback-controlled arsR-egfp circuit on pPR-arsR-ABS-egfp. NFU, culture density normalized fluorescence after 120 min induction time using fluorimeter measurements. Data symbols represent the average from independent biological triplicates. Whiskers, SD (when not visible lay within the symbol size).

Fig. S5. Time response kinetics of the EGFP fluorescence signal in E. coli MG1655 carrying the different feedback and uncoupled bioreporter circuits, at different arsenite concentrations between 0 and 20 μg l−1 and measured in fluorimetry. NFU, culture density normalized fluorescence. Data points show triplicate averages ± one SD.

mbt12031-sup-0002-si.xlsx94K

SI File 1. Excel version of the ArsR-Pars models in feedback or uncoupled modes.

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