Funding Information This work was supported by contract CRSI20-122689 from the Swiss National Science Foundation, Sinergia programme, and by a grant from the 7th European Framework Programme (KBBE.2011.5-289326 ST-FLOW).
Tunable reporter signal production in feedback-uncoupled arsenic bioreporters
Article first published online: 15 JAN 2013
© 2013 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 6, Issue 5, pages 503–514, September 2013
How to Cite
Merulla, D., Hatzimanikatis, V. and van der Meer, J. R. (2013), Tunable reporter signal production in feedback-uncoupled arsenic bioreporters. Microbial Biotechnology, 6: 503–514. doi: 10.1111/1751-7915.12031
- Issue published online: 14 AUG 2013
- Article first published online: 15 JAN 2013
- Manuscript Accepted: 10 DEC 2012
- Manuscript Received: 28 MAY 2012
- Swiss National Science Foundation, Sinergia programme. Grant Number: CRSI20-122689
- 7th European Framework Programme. Grant Number: KBBE.2011.5-289326 ST-FLOW
Mathematical model for ArsR circuits.
Table S1. List of all the primers used in the present work showing sequence, length and melting temperature (Tm).
Fig. S1. Nucleotide alignment of the arsRR73 and the chromosomal arsRK12 genes.
Fig. S2. Relevant part of the DNA sequence of the different promoters used for uncoupled expression of arsRR773.
Fig. S3. Relevant construction details of the feedback (A) and uncoupled (B) circuits. Sequences show part of the arsR gene, the various promoters, the ArsR Binding Sites (ABS) and the start of the egfp reporter gene.
Fig. S4. Arsenite-dependent EGFP fluorescence in cultures of E. coli MG1655 with different uncoupled arsR reporter circuits (pAAUN, pLtetOUN, pJJUN, pVUN, pKUN) compared with the feedback-controlled arsR-egfp circuit on pPR-arsR-ABS-egfp. NFU, culture density normalized fluorescence after 120 min induction time using fluorimeter measurements. Data symbols represent the average from independent biological triplicates. Whiskers, SD (when not visible lay within the symbol size).
Fig. S5. Time response kinetics of the EGFP fluorescence signal in E. coli MG1655 carrying the different feedback and uncoupled bioreporter circuits, at different arsenite concentrations between 0 and 20 μg l−1 and measured in fluorimetry. NFU, culture density normalized fluorescence. Data points show triplicate averages ± one SD.
SI File 1. Excel version of the ArsR-Pars models in feedback or uncoupled modes.
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