Funding InformationThis project was supported by the US Department of Energy, Office of Environmental Management as administered by the SRNL Laboratory Directed Research and Development Program (LDRD09060), DOE Grant DE-EE0003152, Office of Fossil Energy – National Energy Technology Laboratory and support from the Economic Development Partnership for Aiken 0026; Edgefield Counties.
Preservation of H2 production activity in nanoporous latex coatings of Rhodopseudomonas palustris CGA009 during dry storage at ambient temperatures
Article first published online: 21 JAN 2013
© 2013 The Authors. Microbial Biotechnology Published by John Wiley & Sons Ltd and Society for Applied Microbiology.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 6, Issue 5, pages 515–525, September 2013
How to Cite
Piskorska, M., Soule, T., Gosse, J. L., Milliken, C., Flickinger, M. C., Smith, G. W. and Yeager, C. M. (2013), Preservation of H2 production activity in nanoporous latex coatings of Rhodopseudomonas palustris CGA009 during dry storage at ambient temperatures. Microbial Biotechnology, 6: 515–525. doi: 10.1111/1751-7915.12032
- Issue published online: 14 AUG 2013
- Article first published online: 21 JAN 2013
- Manuscript Accepted: 8 DEC 2012
- Manuscript Revised: 7 DEC 2012
- Manuscript Received: 24 AUG 2012
- US Department of Energy, Office of Environmental Management. Grant Number: LDRD09060
- DOE. Grant Number: DE-EE0003152
- Office of Fossil Energy – National Energy Technology Laboratory
- Economic Development Partnership for Aiken 0026; Edgefield Counties
To assess the applicability of latex cell coatings as an ‘off-the-shelf’ biocatalyst, the effect of osmoprotectants, temperature, humidity and O2 on preservation of H2 production in Rhodopseudomonas palustris coatings was evaluated. Immediately following latex coating coalescence (24 h) and for up to 2 weeks of dry storage, rehydrated coatings containing different osmoprotectants displayed similar rates of H2 production. Beyond 2 weeks of storage, sorbitol-treated coatings lost all H2 production activity, whereas considerable H2 production was still detected in sucrose- and trehalose-stabilized coatings. The relative humidity level at which the coatings were stored had a significant impact on the recovery and subsequent rates of H2 production. After 4 weeks storage under air at 60% humidity, coatings produced only trace amounts of H2 (0–0.1% headspace accumulation), whereas those stored at < 5% humidity retained 27–53% of their H2 production activity after 8 weeks of storage. When stored in argon at < 5% humidity and room temperature, R. palustris coatings retained full H2 production activity for 3 months, implicating oxidative damage as a key factor limiting coating storage. Overall, the results demonstrate that biocatalytic latex coatings are an attractive cell immobilization platform for preservation of bioactivity in the dry state.