These authors have equally contributed to this work.
Tight coupling of polymerization and depolymerization of polyhydroxyalkanoates ensures efficient management of carbon resources in Pseudomonas putida
Article first published online: 28 FEB 2013
© 2013 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 6, Issue 5, pages 551–563, September 2013
How to Cite
Arias, S., Bassas-Galia, M., Molinari, G. and Timmis, K. N. (2013), Tight coupling of polymerization and depolymerization of polyhydroxyalkanoates ensures efficient management of carbon resources in Pseudomonas putida. Microbial Biotechnology, 6: 551–563. doi: 10.1111/1751-7915.12040
Funding Information The presented study arose from a larger project within the scope of a patent value fund. In this context we acknowledge the financial support by the Dritte Patentportfolio Beteiligungsgesellschaft mbH & Co. KG (Germany) and the overall project coordination provided by Clou Partners (Germany).
- Issue published online: 14 AUG 2013
- Article first published online: 28 FEB 2013
- Manuscript Accepted: 12 JAN 2013
- Manuscript Revised: 15 NOV 2012
- Manuscript Received: 18 AUG 2012
- Dritte Patentportfolio Beteiligungsgesellschaft mbH & Co. KG (Germany)
Fig. S1. Genetic organization of the pha cluster and the expression system used for the hyperexpression of PhaC2 in PpU 10-33.
Fig. S2. Expression of the phaF, phaI and phaD genes and PHA accumulation in P. putida U.
Fig. S3. Expression of fadD1 and fadD2 genes and PHA accumulation in P. putida U.
Table S1. Biomass and PHA production in P. putida U and the recombinant strains.
Table S2. Strains, mutants and plasmids used in this work.
Table S3. List of oligonucleotides used for the RT-PCR assay in this study.
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