The SuperChip for microbial community structure, and function from all environments

Authors

  • Terry C. Hazen

    Corresponding author
    1. Biological Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA
    • Departments of Civil & Environmental Engineering, Earth & Planetary Sciences, Microbiology, University of Tennessee, Knoxville, TN, USA
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  • Funding Information We are indeed grateful for the support that we have received for the past several years from the ENIGMA programme at Lawrence Berkeley National Laboratory under contract No. DE-AC02-05CH11231 with the Office of Science, Office of Biological and Environmental Research, of the US Department of Energy as part of ENIGMA from the Office of Biological and Environmental Science and in part by contract No. DE-AC05-00OR22725 to Oak Ridge National Laboratory from the US Department of Energy.

For correspondence. E-mail tchazen@utk.edu; Tel. (+1) 865 974 7709; Fax (+1) 865 974 8086.

Summary

We have the technology and capability to develop an all-in-one microarray that can provide complete information on a microbial community, including algae, protozoa, bacteria, archaea, fungi, viruses, antimicrobial resistance, biotoxins and functional activity. With lab-on-a-chip, nanotechnology integrating a variety of the latest methods for a large number of sample types (water, sediment, waste water, food, blood, etc.) it is possible to make a desktop instrument that would have universal applications. There are two major thrusts to this grand challenge that will allow us to take advantage of the latest biotechnological breakthroughs in real time. The first is a bioengineering thrust that will take advantage of the large multidisciplinary laboratories in developing key technologies. Miniaturization will reduce reagent costs and increase sensitivity and reaction kinetics for rapid turnaround time. New and evolving technologies will allow us to port the designs for state-of-the-art microarrays today to completely new nanotechnology inspired platforms as they mature. The second thrust is in bioinformatics to use our existing expertise to take advantage of the rapidly evolving landscape of bioinformatics data. This increasing capacity of the data set will allow us to resolve microbial species to greatly improved levels and identify functional genes beyond the hypothetical protein level. A cheap and portable assay would impact countless areas, including clean water technologies, emerging diseases, bioenergy, infectious disease diagnosis, climate change, food safety, environmental clean-up and bioterrorism. In my opinion it is possible but it will require a very large group of multidiscplenary scientists from multiple institutions crossing many international boundaries and funding over a 5-year period of more than $100 million. Given the impact that this SuperChip could have it is well worth the price!!!

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