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Assessing bacterial diversity in a seawater-processing wastewater treatment plant by 454-pyrosequencing of the 16S rRNA and amoA genes
Article first published online: 10 APR 2013
© 2013 The Authors. Microbial Biotechnology Published by John Wiley & Sons Ltd and Society for Applied Microbiology.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Thematic Issue: Ecological Engineering of the Intestinal Microbiome Connecting the Environment and Food to Therapy and Health
Volume 6, Issue 4, pages 435–442, July 2013
How to Cite
Sánchez, O., Ferrera, I., González, J. M. and Mas, J. (2013), Assessing bacterial diversity in a seawater-processing wastewater treatment plant by 454-pyrosequencing of the 16S rRNA and amoA genes. Microbial Biotechnology, 6: 435–442. doi: 10.1111/1751-7915.12052
Funding Information This work was supported by the Spanish projects Consolider TRAGUA (CSD2006-00044) and CTQ2009-14390-C02-02.
- Issue published online: 11 JUN 2013
- Article first published online: 10 APR 2013
- Manuscript Accepted: 22 FEB 2013
- Manuscript Received: 21 FEB 2013
- Consolider TRAGUA. Grant Numbers: CSD2006-00044, CTQ2009-14390-C02-02
The bacterial community composition of activated sludge from a wastewater treatment plant (Almería, Spain) with the particularity of using seawater was investigated by applying 454-pyrosequencing. The results showed that Deinococcus-Thermus, Proteobacteria, Chloroflexi and Bacteroidetes were the most abundant retrieved sequences, while other groups, such as Actinobacteria, Chlorobi, Deferribacteres, Firmicutes, Planctomycetes, Spirochaetes and Verrumicrobia were reported at lower proportions. Rarefaction analysis showed that very likely the diversity is higher than what could be described despite most of the unknown microorganisms probably correspond to rare diversity. Furthermore, the majority of taxa could not be classified at the genus level and likely represent novel members of these groups. Additionally, the nitrifiers in the sludge were characterized by pyrosequencing the amoA gene. In contrast, the nitrifying bacterial community, dominated by the genera Nitrosomonas, showed a low diversity and rarefaction curves exhibited saturation. These results suggest that only a few populations of low abundant but specialized bacteria are responsible for removal of ammonia in these saline wastewater systems.