Funding Information This research was supported through the EU project ‘Microbial Marine Communities Diversity: from Culture to Function’ (MIRACLE) (EVK3-CT-2002-00087) and the Natural Environment Research Council (UK)-funded grants ‘Isolation of single microbial cells’ (NE/B505770/1) and ‘Novel marine microbial enzymes for use in biocatalysis’ (NE/F014406/1).
The biodiscovery potential of marine bacteria: an investigation of phylogeny and function
Version of Record online: 4 APR 2013
© 2013 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Thematic Issue: Ecological Engineering of the Intestinal Microbiome Connecting the Environment and Food to Therapy and Health
Volume 6, Issue 4, pages 361–370, July 2013
How to Cite
Mühling, M., Joint, I. and Willetts, A. J. (2013), The biodiscovery potential of marine bacteria: an investigation of phylogeny and function. Microbial Biotechnology, 6: 361–370. doi: 10.1111/1751-7915.12054
- Issue online: 11 JUN 2013
- Version of Record online: 4 APR 2013
- Manuscript Accepted: 26 FEB 2013
- Manuscript Revised: 18 FEB 2013
- Manuscript Received: 10 DEC 2012
- EU. Grant Number: EVK3-CT-2002-00087
- Natural Environment Research Council (UK). Grant Numbers: NE/B505770/1, NE/F014406/1
Text S1. Details on enzyme screens.
Table S1. Summary of the bacterial diversity of the 374 marine isolates tested in this study. Identification was based on sequence comparison of a fragment of the 16S rRNA gene with the non-redundant nucleotide (nr/nt) database of the National Center for Bioinformatic Information (NCBI) using the Basic Local Alignment Search Tool (blast: blastn). The closest hits to known genera are given, independent of the level of sequence identity; the exception is one group of isolates that have close similarity to the 16S rRNA gene sequence of Arctic sea ice bacterium ARK10115 (Accession No. AF468367), but no meaningful similarity to any known genus that would allow prediction of their taxonomic identity.
Table S2. Summary of 34 enzymatic activities tested in the 374 marine isolates. The results are depicted as binary data whereby ‘1’ and ‘0’ code for presence and absence respectively.
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