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mbt12057-sup-0001-AppendixS1.doc1118K

Appendix S1. Detailed characterization of all purified bulk soil, rhizosphere soil and B. napus root isolates collected at the control field (BS-CO, RS-CO and R-CO respectively) and the contaminated field (BS-TE, RS-TE and R-TE respectively). The presence of each strain is shown as relative abundances, expressed in percentages, of the total number of colony-forming units per gram fresh weight (cfu gFW−1) bulk soil (BS), rhizosphere soil (RS) or roots (R). Strains are identified to the genus level, their accession numbers as well as their presence in the first, second or third replicate (repl) are displayed. Their potential plant growth promoting (PGP) characteristics are indicated by + when positive and by ++(+) in case of a strong positive test. Bacterial strains testing negative for a phenotypic test were labelled by a – symbol and those not applicable for the test by ‘not detected’ (nd). The PGP characteristics tested were Cd (0.8 and 1.6 mM) and Zn (0.6, 1.0 and 2.5 mM) tolerance and the capacity to solubilize phosphorus (P sol), fixate nitrogen (N2 fix) and produce siderophores (SID), organic acids (OA), ACC deaminase (ACC), indole-3-acetic acid (IAA) and acetoin.

mbt12057-sup-0002-AppendixS2.tif318K

Appendix S2. The neighbour-joining tree of all strains with a different identification (accession number) clustered strains belonging to the same genus together. Sequences were aligned and used for constructing a neighbour-joining tree with PAUP*4.0b10, using default settings. In order to assess branch supports, bootstrap values were calculated with 2000 pseudoreplicates.

mbt12057-sup-0003-AppendixS3.tif3533K

Appendix S3. Total Cd and Zn concentrations {mg [kg dry weight (DW)]−1} in roots, stems, leaves and seeds of B. napus grown at the control field (CO-F) (A and B respectively) and the contaminated field (TE-F) (C and D respectively). Values are means ± standard error of three biological independent replicates (significance levels: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001). Trace element contents measured in different plant parts were compared using two-way anova (plant part and field) and post hoc multiple comparison testing (Tukey Kramer).

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