These authors contributed equally to this work.
Comparative genome analysis of Lactobacillus casei strains isolated from Actimel and Yakult products reveals marked similarities and points to a common origin
Article first published online: 1 JUL 2013
© 2013 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 6, Issue 5, pages 576–587, September 2013
How to Cite
Douillard, F. P., Kant, R., Ritari, J., Paulin, L., Palva, A. and de Vos, W. M. (2013), Comparative genome analysis of Lactobacillus casei strains isolated from Actimel and Yakult products reveals marked similarities and points to a common origin. Microbial Biotechnology, 6: 576–587. doi: 10.1111/1751-7915.12062
Funding Information The present work was supported by the Center of Excellence in Microbial Food Safety Research (Academy of Finland, Grant 141140), Grant ERC 250172 – Microbes Inside from the European Research Council and Grants 137389 and 141130 from the Academy of Finland. F.P.D. was funded by a postdoctoral research fellowship (Academy of Finland, Grant 252123).
- Issue published online: 14 AUG 2013
- Article first published online: 1 JUL 2013
- Manuscript Accepted: 20 MAR 2013
- Manuscript Revised: 15 MAR 2013
- Manuscript Received: 7 FEB 2013
- Center of Excellence in Microbial Food Safety Research (Academy of Finland. Grant Number: 141140
- European Research Council. Grant Number: ERC 250172
- Academy of Finland. Grant Numbers: 137389, 141130, 252123
Vol. 7, Issue 1, 85, Article first published online: 10 DEC 2013
The members of the Lactobacillus genus are widely used in the food and feed industry and show a remarkable ecological adaptability. Several Lactobacillus strains have been marketed as probiotics as they possess health-promoting properties for the host. In the present study, we used two complementary next-generation sequencing technologies to deduce the genome sequences of two Lactobacillus casei strains LcA and LcY, which were isolated from the products Actimel and Yakult, commercialized as probiotics. The LcA and LcY draft genomes have, respectively, an estimated size of 3067 and 3082 Mb and a G+C content of 46.3%. Both strains are close to identical to each other and differ by no more than minor chromosomal re-arrangements, substitutions, insertions and deletions, as evident from the verified presence of one insertion-deletion (InDel) and only 29 single-nucleotide polymorphisms (SNPs). In terms of coding capacity, LcA and LcY are predicted to encode a comparable exoproteome, indicating that LcA and LcY are likely to establish similar interactions with human intestinal cells. Moreover, both L. casei LcA and LcY harboured a 59.6 kb plasmid that shared high similarities with plasmids found in other L. casei strains, such as W56 and BD-II. Further analysis revealed that the L. casei plasmids constitute a good evolution marker within the L. casei species. The plasmids of the LcA and LcY strains are almost identical, as testified by the presence of only three verified SNPs, and share a 3.5 kb region encoding a remnant of a lactose PTS system that is absent from the plasmids of W56 and BD-II but conserved in another smaller L. casei plasmid (pLC2W). Our observations imply that the results obtained in animal and human experiments performed with the Actimel and Yakult strains can be compared with each other as these strains share a very recent common ancestor.