These authors contributed equally to this work.
Engineering of recombinant Escherichia coli cells co-expressing poly-γ-glutamic acid (γ-PGA) synthetase and glutamate racemase for differential yielding of γ-PGA
Article first published online: 6 AUG 2013
© 2013 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Thematic Issue on Biomaterials
Volume 6, Issue 6, pages 675–684, November 2013
How to Cite
Cao, M., Geng, W., Zhang, W., Sun, J., Wang, S., Feng, J., Zheng, P., Jiang, A. and Song, C. (2013), Engineering of recombinant Escherichia coli cells co-expressing poly-γ-glutamic acid (γ-PGA) synthetase and glutamate racemase for differential yielding of γ-PGA. Microbial Biotechnology, 6: 675–684. doi: 10.1111/1751-7915.12075
Funding Information This study was financially supported by the National Key Basic Research Program of China (‘973’ Program) 2012CB725204, the National High Technology Research and Development Program of China (‘863’ Program) 2012AA021505, and the Natural Science Foundation of China Grant Nos. 31070039, 31170030 and 51073081.
- Issue published online: 11 OCT 2013
- Article first published online: 6 AUG 2013
- Manuscript Accepted: 21 JUN 2013
- Manuscript Revised: 19 JUN 2013
- Manuscript Received: 22 APR 2013
- National Key Basic Research Program of China (‘973’ Program). Grant Number: 2012CB725204
- National High Technology Research and Development Program of China (‘863’ Program). Grant Number: 2012AA021505
- Natural Science Foundation of China. Grant Numbers: 31070039, 31170030, 51073081
Fig. S1. d-glutamate content measurement of γ-PGA produced by the four E. coli recombinant strains using the reversed phase HPLC method.
Table S1. The comparison of PgsBCA synthetase complex and RacE from NK-03 and LL3.
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