Recombinant DNA production of spider silk proteins


  • Funding Information The authors thank the NIH (EB014976, EB002520; EY020856; DE017207; EB014283), the National Council for Scientific and Technological Development, Embrapa Genetics Resources and Biotechnology, and Foundation for Research Support, Brasilia, DF, Brazil for support of the studies reviewed here.

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Spider dragline silk is considered to be the toughest biopolymer on Earth due to an extraordinary combination of strength and elasticity. Moreover, silks are biocompatible and biodegradable protein-based materials. Recent advances in genetic engineering make it possible to produce recombinant silks in heterologous hosts, opening up opportunities for large-scale production of recombinant silks for various biomedical and material science applications. We review the current strategies to produce recombinant spider silks.