Recombinant DNA production of spider silk proteins

Authors


  • Funding Information The authors thank the NIH (EB014976, EB002520; EY020856; DE017207; EB014283), the National Council for Scientific and Technological Development, Embrapa Genetics Resources and Biotechnology, and Foundation for Research Support, Brasilia, DF, Brazil for support of the studies reviewed here.

For correspondence. E-mail david.Kaplan@tufts.edu; Tel. (+617) 626 3251; Fax (+617) 627 3231.

Abstract

Spider dragline silk is considered to be the toughest biopolymer on Earth due to an extraordinary combination of strength and elasticity. Moreover, silks are biocompatible and biodegradable protein-based materials. Recent advances in genetic engineering make it possible to produce recombinant silks in heterologous hosts, opening up opportunities for large-scale production of recombinant silks for various biomedical and material science applications. We review the current strategies to produce recombinant spider silks.

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