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mbt212091-sup-0001-si.pptx107K

Fig. S1. Genetic comparison of T3SSs from the Ysc family.

Comparison of the organization of T3SS clusters in A. salmonicida subsp. salmonicida A449, A. hydrophila SSU, A. veronii AER39, A. diversa 2478-85, Pseudomonas aeruginosa PAO1, Photobacterium damselae subsp. damselae CIP 102761, Photorhabdus luminescens subsp. laumondii TTO1, Yersinia pestis biovar Antiqua str. E1979001, and Vibrio parahaemolyticus RIMD 2210633.

mbt212091-sup-0002-si.xls3487K

Table S1. Profound analysis and comparison of published Aeromonas genomes.

Grey: conserved ORFs; light green: ORFs specific of the species; yellow: IS630; pink: other IS elements; red: putative or characterized virulence factors; mauve: ORFs for resistance to antibiotic or heavy metal; dark green: ORFs associated to pili, fimbriae or flagella; blue: ORFs associated with phage; cyan: tRNA and rRNA; orange: ORFs with homology to eukaryotic genes. Aeromonas salmonicida subsp. salmonicida A449, A. salmonicida subsp. achromogenes AS03, A. salmonicida (ATCC7966, ML09-119 and SSU), A. caviae Ae398, A. veronii (B565, AMC34, AMC35, AER39 and AER397), A. aquariorum AAK1, and A. diversa 2478-85.

mbt212091-sup-0003-si.zip13862K

Movies S1 and S2. Rapid cell-rounding induced by the T3SS of A. salmonicida.

Epithelioma papulosum cyprini cells (EPCs) seeded in a glass bottom well and exposed to A. salmonicida wt JF2267 (S1) and ΔascV mutant JF2747 (S2) expressing GFP (after induction with IPTG) for 120 min. Following infection at 20°C, time-lapse imaging using video microscopy was performed using a Nikon Eclipse TE2000-U inverted microscope equipped with a climate-controlled chamber. Data acquisition and image processing were performed using NIS software of Nikon Instruments. DIC and fluorescence images were acquired and assembled in movies. Image acquisition at intervals of 2 min for 120 min. MOI of 10:1 (bacteria : fish cells).

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