L. T.-G and H. G.-A. have contributed equally to this paper.
Combination of degradation pathways for naphthalene utilization in Rhodococcus sp. strain TFB
Article first published online: 11 DEC 2013
© 2013 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 7, Issue 2, pages 100–113, March 2014
How to Cite
Tomás-Gallardo, L., Gómez-Álvarez, H., Santero, E. and Floriano, B. (2014), Combination of degradation pathways for naphthalene utilization in Rhodococcus sp. strain TFB. Microbial Biotechnology, 7: 100–113. doi: 10.1111/1751-7915.12096
Funding Information Work in the authors laboratory was supported by the Spanish Ministry of Economy and Competitivity, grants BIO2011-24003 and CSD2007-00005, and by the Andalusian Government, grants P05-CVI-131 and P07-CVI-2518.
- Issue published online: 14 FEB 2014
- Article first published online: 11 DEC 2013
- Manuscript Accepted: 7 OCT 2013
- Manuscript Revised: 3 OCT 2013
- Manuscript Received: 28 JUL 2013
- Spanish Ministry of Economy and Competitivity. Grant Numbers: BIO2011-24003, CSD2007-00005
- Andalusian Government. Grant Numbers: P05-CVI-131, P07-CVI-2518
Rhodococcus sp. strain TFB is a metabolic versatile bacterium able to grow on naphthalene as the only carbon and energy source. Applying proteomic, genetic and biochemical approaches, we propose in this paper that, at least, three coordinated but independently regulated set of genes are combined to degrade naphthalene in TFB. First, proteins involved in tetralin degradation are also induced by naphthalene and may carry out its conversion to salicylaldehyde. This is the only part of the naphthalene degradation pathway showing glucose catabolite repression. Second, a salicylaldehyde dehydrogenase activity that converts salicylaldehyde to salicylate is detected in naphthalene-grown cells but not in tetralin- or salicylate-grown cells. Finally, we describe the chromosomally located nag genes, encoding the gentisate pathway for salicylate conversion into fumarate and pyruvate, which are only induced by salicylate and not by naphthalene. This work shows how biodegradation pathways in Rhodococcus sp. strain TFB could be assembled using elements from different pathways mainly because of the laxity of the regulatory systems and the broad specificity of the catabolic enzymes.