Weitao Geng and Chao Yang contributed equally to this work.
Cloning of ε-poly-L-lysine (ε-PL) synthetase gene from a newly isolated ε-PL-producing Streptomyces albulus NK660 and its heterologous expression in Streptomyces lividans
Version of Record online: 14 JAN 2014
© 2014 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 7, Issue 2, pages 155–164, March 2014
How to Cite
Geng, W., Yang, C., Gu, Y., Liu, R., Guo, W., Wang, X., Song, C. and Wang, S. (2014), Cloning of ε-poly-L-lysine (ε-PL) synthetase gene from a newly isolated ε-PL-producing Streptomyces albulus NK660 and its heterologous expression in Streptomyces lividans. Microbial Biotechnology, 7: 155–164. doi: 10.1111/1751-7915.12108
Funding Information The authors gratefully acknowledge the financial support from National Key Basic Research Program of China (‘973’ Program, 2012CB725204), National High Technology Research and Development Program of China (‘863’ Program, 2012AA021505), Natural Science Foundation of China (grant nos. 31070039, 31170030, 51073081 and 31300032), Project of Tianjin, China (grant no. 13JCQNJC09700).
- Issue online: 14 FEB 2014
- Version of Record online: 14 JAN 2014
- Manuscript Accepted: 16 DEC 2013
- Manuscript Revised: 12 DEC 2013
- Manuscript Received: 4 OCT 2013
- National Key Basic Research Program of China. Grant Number: 2012CB725204
- National High Technology Research and Development Program of China. Grant Number: 2012AA021505
- Natural Science Foundation of China. Grant Numbers: 31070039, 31170030, 51073081, 31300032
- Project of Tianjin, China. Grant Number: 13JCQNJC09700
Fig. S1. A pair of primers designed for amplifying the partial pls gene of S. albulus NK660.
Fig. S2. Detection of ε-PL production in S. lividans by Dragendorff reagent.
Fig. S3. A. 1H NMR spectrum of a reference standard of ε-PL.
B. 13C NMR spectrum of a reference standard of ε-PL.
Fig. S4. A. The construction of pHZ-pls confirmed by restriction enzyme digestion.
B. The PCR detection of the pHZ-pls in S. lividans ZX7.
Table S1. Culture characteristics of S. albulus NK660.
Table S2. Effects of different carbon sources on cell dry weight, final pH and ε-PL yield.
Table S3. The primers used for cloning of the pls gene from S. albulus NK660 by genome walking.
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