Funding Information This work is supported by the National Science Foundation grant to J. R. S. (NSF-MUSES 0628282) and a Dorthy Bertine Internship award to J. Z. L. from the Sussman Fund.
Microbial response to single-cell protein production and brewery wastewater treatment
Article first published online: 16 MAY 2014
© 2014 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 8, Issue 1, pages 65–76, January 2015
How to Cite
Lee, J. Z., Logan, A., Terry, S. and Spear, J. R. (2015), Microbial response to single-cell protein production and brewery wastewater treatment. Microbial Biotechnology, 8: 65–76. doi: 10.1111/1751-7915.12128
- Issue published online: 28 JAN 2015
- Article first published online: 16 MAY 2014
- Manuscript Accepted: 28 MAR 2014
- Manuscript Revised: 20 MAR 2014
- Manuscript Received: 20 OCT 2013
- National Science Foundation. Grant Number: 0628282
- Dorthy Bertine Internship
As global fisheries decline, microbial single-cell protein (SCP) produced from brewery process water has been highlighted as a potential source of protein for sustainable animal feed. However, biotechnological investigation of SCP is difficult because of the natural variation and complexity of microbial ecology in wastewater bioreactors. In this study, we investigate microbial response across a full-scale brewery wastewater treatment plant and a parallel pilot bioreactor modified to produce an SCP product. A pyrosequencing survey of the brewery treatment plant showed that each unit process selected for a unique microbial community. Notably, flow equalization basins were dominated by Prevotella, methanogenesis effluent had the highest levels of diversity, and clarifier wet-well samples were sources of sequences for the candidate bacterial phyla of TM7 and BD1-5. Next, the microbial response of a pilot bioreactor producing SCP was tracked over 1 year, showing that two different production trials produced two different communities originating from the same starting influent. However, SCP production resulted generally in enrichment of several clades of rhizospheric diazotrophs of Alphaproteobacteria and Betaproteobacteria in the bioreactor and even more so in the final product. These diazotrophs are potentially useful as the basis of a SCP product for commercial feed production.