Validation of a cheap and simple nondestructive method for obtaining AFLPs and DNA sequences (mitochondrial and nuclear) in amphibians
Article first published online: 15 SEP 2012
© 2012 Blackwell Publishing Ltd
Molecular Ecology Resources
Volume 12, Issue 6, pages 1090–1096, November 2012
How to Cite
Gallardo, C. E., Correa, C., Morales, P., Sáez, P. A., Pastenes, L. & Méndez, M. A. (2012). Validation of a cheap and simple nondestructive method for obtaining AFLPs and DNA sequences (mitochondrial and nuclear) in amphibians. Molecular Ecology Resources, 12: 1090–1096. doi: 10.1111/1755-0998.12013
- Issue published online: 11 OCT 2012
- Article first published online: 15 SEP 2012
- Manuscript Accepted: 14 AUG 2012
- Manuscript Revised: 7 AUG 2012
- Manuscript Received: 4 JUL 2011
Table S1 Quantification of the amount of DNA obtained for each individual from the samples of buccal swabs and interdigital membranes (or toe-clipped in the case of Pleurodema thaul). Code sample of Herpetological Collection of the Departamento de Biología Celular y Genética de la Universidad de Chile (DBGUCH).
Table S2 AFLP protocols for Rhinella spinulosa, R. atacamensis, Pleurodema thaul and Telmatobius. One-Phor-All buffer plus (10X) = 100 mm Tris–acetate, pH 7.5, 100 mm Mg-acetate, 500 mm K-acetate. NEB = New England Biolabs. Cf = final concentration.
Table S3 Primer combinations used in selective PCRs in the AFLP protocol for Rhinella spinulosa, R. atacamensis, Pleurodema thaul and Telmatobius. The range of fragment sizes assessed in the profiles obtained with each combination is indicated.
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