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men12015-sup-0001-AppendixS1-S4.pdfapplication/PDF737KAppendix S1 Formal demonstration of the existence of 0, 1 or two solutions in the VO system of equations. Appendix S2 Distribution of null allele frequency used in the simulations. Appendix S3 Method for the numerical optimization of the externally supplied F-estimate. Appendix S4 Comparison of the methods: the modified VO method (VOm) of Van Oosterhout et al. 2006; and the CB method of Chybicki & Burczyk 2009.

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