Improving PCR detection of prey in molecular diet studies: importance of group-specific primer set selection and extraction protocol performances
Article first published online: 8 NOV 2012
© 2012 Blackwell Publishing Ltd
Molecular Ecology Resources
Volume 13, Issue 1, pages 117–127, January 2013
How to Cite
Zarzoso-Lacoste, D., Corse, E. and Vidal, E. (2013), Improving PCR detection of prey in molecular diet studies: importance of group-specific primer set selection and extraction protocol performances. Molecular Ecology Resources, 13: 117–127. doi: 10.1111/1755-0998.12029
- Issue published online: 11 DEC 2012
- Article first published online: 8 NOV 2012
- Manuscript Accepted: 20 SEP 2012
- Manuscript Revised: 16 SEP 2012
- Manuscript Received: 1 MAR 2012
Additional File S1. List of Plant and Animal samples collected on Niau Island to Constitute the DNA template of our prey bank.
Additional File S2. List and source of tested primers.
Additional File S3. Extraction protocol details.
Additional File S4. Results of cross-amplification tests and selection criteria of group-specific primer pairs.
Additional File S5. Plant rbcL sequences amplified from rat diet samples.
Additional File S6. Results of the morphological analysis of rats' stomach and fecal samples.
Additional File S7. Values of both DNA concentration (ng/mL) and A260/A280 measured in both stomach (ST) and fecal (F) samples for all the tested Extraction Protocols; EP1: QIAamp DNA stool mini kit, EP2: DNeasy mericon food, EP3: CTAB, EP4: CTAB + QIAquick purification. Re 1 to 7: Rattus exulansn°1–7 and Rr 1 to 5: Rattus rattus n°1–5.
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