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Fig. S1 Locations of the four populations of Populus euphratica in this research. The black dots indicate the pop code as described in the Table 1.

Table S1. The information of the 56 candidate gSSR loci, not including the loci used in the multiplex PCR kit-1. All the information based on the simplex PCR and sequencing experiment in Populus euphratica.

Table S2. The information of the 31 candidate eSSR loci, the loci in the multiplex PCR kit-2 were not included.

Table S3. Results of the transferability in the five sections of Poplar.

Table S4. Hardy-Weinberg equilibrium in the four populations of Populus euphratica.

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