Direct sequencing of haplotypes from diploid individuals through a modified emulsion PCR-based single-molecule sequencing approach
Article first published online: 11 DEC 2012
© 2012 Blackwell Publishing Ltd
Molecular Ecology Resources
Volume 13, Issue 1, pages 135–143, January 2013
How to Cite
Metzger, B. P. H., Gelembiuk, G. W. and Lee, C. E. (2013), Direct sequencing of haplotypes from diploid individuals through a modified emulsion PCR-based single-molecule sequencing approach. Molecular Ecology Resources, 13: 135–143. doi: 10.1111/1755-0998.12034
- Issue published online: 11 DEC 2012
- Article first published online: 11 DEC 2012
- Manuscript Accepted: 11 OCT 2012
- Manuscript Revised: 8 OCT 2012
- Manuscript Received: 7 AUG 2012
- NSF. Grant Number: DEB-0745828
- University of Wisconsin
Figure S1 Theoretical probability that a single vesicle contains either zero or one copy of a specific haplotype as a function of the total number of vesicles and the total number of copies of that haplotype.
Figure S2 Haplotype sequences of a diploid individual generated using conventional PCR (a) and our modified single-molecule sequencing approach (b–m).
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