Cold Code: the global initiative to DNA barcode amphibians and nonavian reptiles

Authors

  • Robert W. Murphy,

    Corresponding author
    1. Centre for Biodiversity and Conservation Biology, Royal Ontario Museum, Toronto, Canada
    • State Key Laboratory of Genetic Resources and Evolution State, and Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China
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  • Andrew J. Crawford,

    1. Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia
    2. Smithsonian Tropical Research Institute, Panamá, Republic of Panama
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  • Aaron M. Bauer,

    1. Department of Biology, Villanova University, Villanova, PA, USA
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  • Jing Che,

    1. State Key Laboratory of Genetic Resources and Evolution State, and Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China
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  • Stephen C. Donnellan,

    1. Evolutionary Biology Unit, South Australian Museum, Adelaide, SA, Australia
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  • Uwe Fritz,

    1. Museum of Zoology, Dresden, Germany
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  • Célio F.B. Haddad,

    1. Departmento de Zoologia, Instituto de Biociências, Universidade Estadual Paulista, Rio Claro, São Paulo, Brazil
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  • Zoltán T. Nagy,

    1. Joint Experimental Molecular Unit, Royal Belgian Institute of Natural Sciences, Brussels, Belgium
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  • Nikolay A. Poyarkov,

    1. Department of Vertebrate Zoology, Moscow MV Lomonosov State University, Moscow, Russia
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  • Miguel Vences,

    1. Zoological Institute, Technical University of Braunschweig, Braunschweig, Germany
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  • Wen-zhi Wang,

    1. State Key Laboratory of Genetic Resources and Evolution State, and Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China
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  • Ya-ping Zhang

    1. State Key Laboratory of Genetic Resources and Evolution State, and Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China
    2. Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming, China
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Correspondence: Robert W. Murphy, Fax: 1-416-586-5553; E-mail: bob.murphy@utoronto.ca

Abstract

DNA barcoding facilitates the identification of species and the estimation of biodiversity by using nucleotide sequences, usually from the mitochondrial genome. Most studies accomplish this task by using the gene encoding cytochrome oxidase subunit I (COI; Entrez COX1). Within this barcoding framework, many taxonomic initiatives exist, such as those specializing in fishes, birds, mammals, and fungi. Other efforts center on regions, such as the Arctic, or on other topics, such as health. DNA barcoding initiatives exist for all groups of vertebrates except for amphibians and nonavian reptiles. We announce the formation of Cold Code, the international initiative to DNA barcode all species of these ‘cold-blooded’ vertebrates. The project has a Steering Committee, Coordinators, and a home page. To facilitate Cold Code, the Kunming Institute of Zoology, Chinese Academy of Sciences will sequence COI for the first 10 specimens of a species at no cost to the steward of the tissues.

Ancillary