Molecular identification of dipteran pests (Diptera: Sciaroidea) from shiitake mushroom

Authors

  • Seunggwan Shin,

    1. Insect Biosystematics Laboratory, Seoul National University, Seoul, Korea
    2. Research Institute for Agricultural and Life Sciences, Seoul National University, Seoul, Korea
    3. Department of Agricultural Biotechnology, Seoul National University, Seoul, Korea
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  • Sunghoon Jung,

    1. Insect Biosystematics Laboratory, Seoul National University, Seoul, Korea
    2. Research Institute for Agricultural and Life Sciences, Seoul National University, Seoul, Korea
    3. Department of Agricultural Biotechnology, Seoul National University, Seoul, Korea
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  • Heungsik Lee,

    1. Central Post-Entry Quarantine Station, Animal Plant and Fisheries Quarantine and Inspection Agency, Suwon, Korea
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  • Seunghwan Lee

    Corresponding author
    1. Research Institute for Agricultural and Life Sciences, Seoul National University, Seoul, Korea
    2. Department of Agricultural Biotechnology, Seoul National University, Seoul, Korea
    • Insect Biosystematics Laboratory, Seoul National University, Seoul, Korea
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Correspondence: Seunghwan Lee, Fax: +82 2 873 2319; E-mail: seung@snu.ac.kr

Abstract

On shiitake farms, mycophagous maggots can cause serious damage by preventing formation of the fruiting body. Recently, these pests have significantly reduced shiitake production in Korea. However, larvae and female adults cannot be identified due to their lack of morphological characteristics. Therefore, farmers and applied entomologists are unable to determine which species is the primary cause of the shiitake damage. In this study, mycophagous flies (colonized larvae) were collected from damaged shiitake farms and subsequently identified by matching identified males with the cytochrome c oxidase subunit I (COI) sequences from the larvae. Divergences of the COI sequences among the species discriminated the clusters clearly, and the mycophagous pests were identified as Camptomyia corticalis and C. heterobia. Interestingly, these two species coexisted under the bark of shiitake oak bed logs.

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