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A NGS approach to the encrusting Mediterranean sponge Crella elegans (Porifera, Demospongiae, Poecilosclerida): transcriptome sequencing, characterization and overview of the gene expression along three life cycle stages

  1. A. R. Pérez-Porro1,2,*,
  2. D. Navarro-Gómez2,
  3. M. J. Uriz1,
  4. G. Giribet2

Article first published online: 26 FEB 2013

DOI: 10.1111/1755-0998.12085

Issue

Molecular Ecology Resources

Molecular Ecology Resources

Volume 13, Issue 3, pages 494–509, May 2013

Additional Information

How to Cite

Pérez-Porro, A. R., Navarro-Gómez, D., Uriz, M. J. and Giribet, G. (2013), A NGS approach to the encrusting Mediterranean sponge Crella elegans (Porifera, Demospongiae, Poecilosclerida): transcriptome sequencing, characterization and overview of the gene expression along three life cycle stages. Molecular Ecology Resources, 13: 494–509. doi: 10.1111/1755-0998.12085

Author Information

  1. 1

    Center for Advanced Studies of Blanes (CEAB-CSIC), Girona, Blanes, Spain

  2. 2

    Museum of Comparative Zoology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA

*Correspondence: Alicia R. Pérez-Porro, Fax: + 1 617-495-5667; E-mail: arodriguezperezporro@g.harvard.edu

Publication History

  1. Issue published online: 12 APR 2013
  2. Article first published online: 26 FEB 2013
  3. Manuscript Accepted: 18 JAN 2013
  4. Manuscript Revised: 16 JAN 2013
  5. Manuscript Received: 8 AUG 2012

Funded by

  • Spanish Ministerio de Economía y Competitividad. Grant Number: CTM2007-66635-C02-01
  • Benthomics. Grant Number: CTM2010-22218-C02-01
  • US National Science Foundation. Grant Numbers: 0531757, 0732903, 0844881

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Table S1 Average coverage statistics for the different assemblies of Crella elegans

Table S3 Number and % of contigs without BLAST hit, with blast hit (but without annotation) and with functional annotation, by size range. (blast performed against the Metazoa + Fungi nr database of NCBI)

Table S4 Number and % of contigs without blast hit, with blast hit (but without annotation) and with functional annotation, by size range. (blast performed against the Porifera nr database of NCBI)

Table S5 OHR (Ortholog hit ratio) statistics for the different assemblies of Crella elegans

Table S6 Identity of the five most redundant proteins in each assembly of Crella elegans

Table S7 Definition (accordingly with NCBI) and RPKM values of the 10 most expressed contigs per life cycle stage

men12085-sup-0002-SupplMatS2.PDFapplication/PDF3496KFig. S2 Log/log plots showing the positive correlation between contig length and the number of reads for a given contig for all four assemblies.

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