men12195-sup-0001-FigS1.tiffTIFF image1521KFig. S1 Flow chart for barcoding ciliates. Single cells are isolated from environmental samples, enrichments or pure cultures, stained with 10% LUGOL solution and photographed under the light microscope. The same individual target cells are destained in sodium thiosulphate for protocol see (Auinger et al. 2008) and subjected to PCR with primers targeting the D1-D2 region of the LSU rDNA. Gene fragments are Sanger-sequenced and deposited as voucher along with other information (Pawlowski et al. 2012).
men12195-sup-0002-TableS1.docxWord document110KTable S1 Ciliates including the D1-primer region or the D2-primer region or the complete D1-D2 fragment of the LSU rDNA and accession numbers available in GenBank (April 30, 2013) and used for in silico primer analysis.

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