Characterizing DNA preservation in degraded specimens of Amara alpina (Carabidae: Coleoptera)
Article first published online: 30 DEC 2013
© 2013 John Wiley & Sons Ltd
Molecular Ecology Resources
Volume 14, Issue 3, pages 606–615, May 2014
How to Cite
Heintzman, P. D., Elias, S. A., Moore, K., Paszkiewicz, K. and Barnes, I. (2014), Characterizing DNA preservation in degraded specimens of Amara alpina (Carabidae: Coleoptera). Molecular Ecology Resources, 14: 606–615. doi: 10.1111/1755-0998.12205
- Issue published online: 11 APR 2014
- Article first published online: 30 DEC 2013
- Accepted manuscript online: 22 NOV 2013 04:50PM EST
- Manuscript Accepted: 19 NOV 2013
- Manuscript Revised: 15 NOV 2013
- Manuscript Received: 20 AUG 2013
- RHUL Reid Scholarship
- RHUL Research Strategy Fund
- SYNTHESYS. Grant Number: SE-TAF-1185
- European Community–Research Infrastructure Action
Data S1 Supplementary methods.
Data S2 Duplicate removal bias.
Fig. S1 Customised workflow for analysis of Illumina paired-end DNA sequence data.
Table S1 Data on all Amara specimens used in this study.
Table S2 Locality data for ancient specimens of A. alpina.
Table S3 Primers used in this study.
Table S4 Details of the 15 reference sequences used to retrieve A. alpina sequences from the merged read files.
Table S5 The proportion of reads per sample (a) aligned to the 15 reference sequences listed in Table S4, and (b) affected by the duplicate removal bias toward nuclear DNA in modern and museum samples.
Table S6 Mean fragment length differences between mitochondrial and nuclear DNA.
Table S7 Details of the contigs used for metagenomic assessment, and the proportion that were taxonomically assigned, including those for the Insecta.
Table S8 Major taxonomic components of the groups identified in Fig 3, and their inferred origin.
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