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men12225-sup-0001-FigureS1-S8.docxWord document486K

Figure. S1 Genotype repeatability between resequenced samples.

Figure. S2 Comparison of Ai in independent replicates.

Figure. S3 Ai distribution across individuals using a low allele validation threshold.

Figure. S4 Raw sequencing depth distribution of variants among amplicons.

Figure. S5 Linear relationship between total amplicon sequencing depth and Ai.

Figure. S6 Confirmation of maximum Ai across samples using PCA.

Figure. S7 Confirmation of Ai estimates among samples.

Figure. S8 Analysis of selection on MHC class IIb sequences of guppies.

men12225-sup-0002-TableS1.xlsxapplication/msexcel42KTable S1 Genotype estimates and confirmation level across seven populations of guppy.
men12225-sup-0003-TableS2.xlsxapplication/msexcel13KTable S2 Comparisons of Ai between CNV model and DOC criterion genotyping method and a commonly used depth allele validation genotyping method.
men12225-sup-0004-TableS3.xlsxapplication/msexcel31KTable S3 Comparisons of Ai, Li and CNV model estimates between resequenced samples.
men12225-sup-0005-AppendixS1.xlsxapplication/msexcel63KAppendix S1 Amplicon sequence data and sample genotypes.
men12225-sup-0006-AppendixS2.xlsmapplication/vnd.ms-excel.sheet.macroenabled.121144KAppendix S2 Excel Macro for genotyping.
men12225-sup-0007-AppendixS3.fastatext/fasta87KAppendix S3 Multiple alignment of allelic variants.
men12225-sup-0008-AppendixS4.r.txtplain text document2KAppendix S4 Code to conduct Principle Components Analysis in R.

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