men12229-sup-0001-FigS1.pdfapplication/PDF36KFig. S1 Results of PCR trials to test primer breadth and selectivity using 37 reference isolates.
men12229-sup-0002-FigS2.pdfapplication/PDF98KFig. S2 Comparison of detection ability of operational taxonomic units (OTUs) identified at 10, 5 and 3% sequence dissimilarity from a DNA mixture of 37 reference isolates subjected to 454 pyrosequencing using the different primer pairs investigated.
men12229-sup-0003-FigS3.pdfapplication/PDF198KFig. S3 Rarefaction curves generated for each primer pair and orchid species (Anacamptis morio, Ophrys tenthredinifera, Serapias lingua) studied.
men12229-sup-0004-TableS1-S3.docxWord document205K

Table S1 Primer sequences used in this studya.

Table S2 In silico PrimerProspector analysis of all GenBank sequences corresponding to 22 orchid-associated fungal families/genera listed in Dearnaley (2007) and Dearnaley et al. (2012).

Table S3 Pyrosequencing fusion primer component sequences and concatemer structurea.

men12229-sup-0005-DataS1.fastext/fas33KData S1 Reference Isolates (ITS-1).fas.
men12229-sup-0006-DataS2.fastext/fas20KData S2 Reference Isolates (ITS-2).fas.
men12229-sup-0007-DataS3.fastext/fas14KData S3 Reference isolates (LSU).fas.
men12229-sup-0008-DataS4.fastext/fas14KData S4 Reference isolates (SSU).fas.
men12229-sup-0009-DataS5.fastext/fas14KData S5 Environmental OTUs (ITS3-ITS4OF).fas.
men12229-sup-0010-DataS6.fastext/fas10KData S6 Environmental OTUs (ITS86F-ITS4).fas.

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