Table S1. The 96 transcriptome-based gene models used for primer development, primers and reference sequences.

Table S2. Testing of 96 primer pairs for cross-amplification for the genus Triturus.

Table S3. Sampling details.

Table S4. Number of reads per individual per marker.

Table S5. Filtered SNP report used to construct consensus sequences.

Table S6. Allelic variants per marker.

Table S7. Data for 132 individual crested newts in genotypic format.

Table S8. Results of the newhybrids analysis.

Table S9. Distribution of 27 markers distinguishing T. ivanbureschi and T. macedonicus in the presumed hybrid population.

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