Parallel tagged amplicon sequencing of transcriptome-based genetic markers for Triturus newts with the Ion Torrent next-generation sequencing platform
Version of Record online: 19 MAR 2014
© 2014 The Authors. Molecular Ecology Resources Published by John Wiley & Sons Ltd.
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Molecular Ecology Resources
Volume 14, Issue 5, pages 1080–1089, September 2014
How to Cite
Wielstra, B., Duijm, E., Lagler, P., Lammers, Y., Meilink, W. R. M., Ziermann, J. M. and Arntzen, J. W. (2014), Parallel tagged amplicon sequencing of transcriptome-based genetic markers for Triturus newts with the Ion Torrent next-generation sequencing platform. Molecular Ecology Resources, 14: 1080–1089. doi: 10.1111/1755-0998.12242
- Issue online: 12 AUG 2014
- Version of Record online: 19 MAR 2014
- Accepted manuscript online: 27 FEB 2014 12:56AM EST
- Manuscript Accepted: 19 FEB 2014
- Manuscript Revised: 15 FEB 2014
- Manuscript Received: 13 DEC 2013
- Naturalis Biodiversity Center
- NWO. Grant Number: 817.02.027
- NBC Naturalis. Grant Number: PS2010.07
Table S1. The 96 transcriptome-based gene models used for primer development, primers and reference sequences.
Table S2. Testing of 96 primer pairs for cross-amplification for the genus Triturus.
Table S3. Sampling details.
Table S4. Number of reads per individual per marker.
Table S5. Filtered SNP report used to construct consensus sequences.
Table S6. Allelic variants per marker.
Table S7. Data for 132 individual crested newts in genotypic format.
Table S8. Results of the newhybrids analysis.
Table S9. Distribution of 27 markers distinguishing T. ivanbureschi and T. macedonicus in the presumed hybrid population.
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