Sequence capture using PCR-generated probes: a cost-effective method of targeted high-throughput sequencing for nonmodel organisms
Article first published online: 7 APR 2014
© 2014 John Wiley & Sons Ltd
Molecular Ecology Resources
Volume 14, Issue 5, pages 1000–1010, September 2014
How to Cite
Peñalba, J. V., Smith, L. L., Tonione, M. A., Sass, C., Hykin, S. M., Skipwith, P. L., McGuire, J. A., Bowie, R. C. K. and Moritz, C. (2014), Sequence capture using PCR-generated probes: a cost-effective method of targeted high-throughput sequencing for nonmodel organisms. Molecular Ecology Resources, 14: 1000–1010. doi: 10.1111/1755-0998.12249
- Issue published online: 12 AUG 2014
- Article first published online: 7 APR 2014
- Accepted manuscript online: 11 MAR 2014 06:36AM EST
- Manuscript Accepted: 12 FEB 2014
- Manuscript Revised: 11 FEB 2014
- Manuscript Received: 8 JUL 2013
- National Science Foundation and the Museum of Vertebrate Zoology
Figure S1 Comparison of coverage and sequence divergence.
Figure S2 Comparison of coverage and probe length.
Figure S3 Mitochondrial Capture Specificity.
Table S1 Locus information for passerine project.
Table S2 Locus information for Draco project.
Table S3 Average coverage per locus for the passerine project (with flanking regions).
Table S4 Average coverage per locus for the Enyalius project (with flanking regions).
Table S5 Average coverage per locus for the Draco project (with flanking regions).
Table S6 Average coverage per locus for the passerine project (without flanking regions).
Table S7 Cost comparisons of SCPP and commercial probe kits.
Table S8 SCPP Reagent Costs – Probe Generation & Hybridization.
Table S9 SCPP Reagent Costs – Oligos.
Table S10 Capture Assessment – qPCR.
Appendix S1 Bioinformatics of read processing and phylogenetic methods for passerine tree.
|men12249-sup-0002-ProtocolS1.pdf||application/PDF||221K||Appendix S2 Detailed protocol for SCPP.|
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