The first three authors contributed equally to this paper.
Correlation between senescence-associated beta-galactosidase expression in articular cartilage and disease severity of patients with knee osteoarthritis
Article first published online: 3 JUN 2013
© 2013 Asia Pacific League of Associations for Rheumatology and Wiley Publishing Asia Pty Ltd
International Journal of Rheumatic Diseases
How to Cite
Gao, S.-G., Zeng, C., Li, L.-J., Luo, W., Zhang, F.-J., Tian, J., Cheng, C., Tu, M., Xiong, Y.-L., Jiang, W., Xu, M. and Lei, G.-H. (2013), Correlation between senescence-associated beta-galactosidase expression in articular cartilage and disease severity of patients with knee osteoarthritis. International Journal of Rheumatic Diseases. doi: 10.1111/1756-185X.12096
- Article first published online: 3 JUN 2013
- National 863 project of China. Grant Number: 2011AA030101
- National Natural Science Foundation of China. Grant Numbers: 81201420, 81272034, 2012QNZT103, 2012QNZT095
- National Clinical Key Department Construction Projects of China. Grant Numbers: 2012QNZT103, 2012QNZT095
- Central Universities in Central South University. Grant Numbers: 2012QNZT103, 2012QNZT095
- articular cartilage;
- senescence-associated beta-galactosidase
The purposes of this study were to investigate senescence-associated beta-galactosidase (SA-beta-Gal) levels in articular cartilage of knee osteoarthritis (OA) and the relationship with severity of the disease.
All the 50 cartilage tissues, including normal (controls) and OA cartilage were ascribed to four groups of normal, mild lesions, moderate lesions and severe lesions on the basis of the modified Mankin score. Immunohistochemistry was used to assess the SA-beta-Gal expression in articular cartilage.
No SA-beta-Gal staining was observed in the normal articular cartilage samples. SA-beta-Gal staining was found in a subset of the chondrocytes close to the lesion site of mild, moderate and severe damaged knee OA cartilage. The percentage of SA-beta-Gal-positive chondrocytes in articular cartilage was 0% in controls, 13.00 ± 5.77% in mild lesions, 31.65 ± 6.91% in moderate lesions and 51.95 ± 6.21% in severe lesions. SA-beta-Gal expression in mild lesions, moderate lesions and severe lesions was higher compared with that of controls (P < 0.0001). SA-beta-Gal expression in moderate lesions and severe lesions were higher with respect to mild lesion samples (P < 0.0001). SA-beta-Gal expression in severe lesions was elevated compared with those of moderate lesions (P < 0.0001). Subsequent analysis showed that articular cartilage SA-beta-Gal levels correlated with severity of disease (Spearman's ρ = 0.94, P < 0.0001).
SA-beta-Gal expression in articular cartilage is associated with progressive knee OA joint damage and is a potential indictor of disease severity.