No evidence of association between CTLA-4 polymorphisms and systemic lupus erythematosus in Iranian patients

Authors

  • Mahdieh Shojaa,

    1. Protection Unit of Clinical Research Center, Golestan University of Medical Sciences, Gorgan, Iran
    2. Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran
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  • Naemeh Javid,

    1. Faculty of Medicine, Department of Microbiology, Infection Disease Research Center, Golestan University of Medical Sciences, Gorgan, Iran
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  • Mahsa Amoli,

    1. Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran
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  • Fatemeh Shakeri,

    1. Department of Microbiology, Payam Noor University of Golestan, Gorgan, Iran
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  • Nader M. Samaei,

    1. Department of Genetics, Congenital Malformations Research Center, OMICS Research Center, Hematology and Oncology Research Center, Golestan University of Medical Sciences, Gorgan, Iran
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  • Mehrdad Aghaie,

    Corresponding author
    1. Faculty of Medicine, Department of Rheumatology, Bone Joint and Connective Tissue Research Center (BJCRC), Golestan University of Medical Sciences, Gorgan, Iran
    • Correspondence: Professor Mehrdad Aghaie, Faculty of Medicine, Department of Rheumatology, Bone Joint and Connective Tissue Research Center (BJCRC), Golestan University of Medical Sciences, Gorgan, Iran. Email: shojaamahdieh@yahoo.com

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  • Patricia Khashayar,

    1. Osteoporosis Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
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  • Sedigheh Livani

    1. Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran
    2. Faculty of Medicine, Department of Microbiology, Infection Disease Research Center, Golestan University of Medical Sciences, Gorgan, Iran
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Abstract

Aim

Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) is an important negative regulator of T-cell responses. CTLA-4 polymorphisms have been confirmed to be associated with several autoimmune diseases such as systemic lupus erythematosus (SLE). We analyzed the role of CTLA-4 polymorphism at positions 1661 and 1722 in Iranian patients suffering from SLE.

Methods

One hundred and eighty SLE patients and 304 ethnically and age-matched healthy controls were studied. Polymerase chain reaction restriction fragments length polymorphism (PCR-RFLP) was used to analyze the genotype and allele frequencies of these polymorphisms.

Results

There was no significant association between the studied genotypic and allelic frequencies between SLE patients and the controls. Although the TC genotype in 1722TC polymorphism was more common among the control group, the correlation was not statistically significant.

Conclusion

Our results suggest that the 1661AG and 1722TC polymorphisms in the promoter region of the CTLA-4 gene does not play any role in genetic susceptibility to SLE. However, further studies on larger sample sizes are needed to approve our results.

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