Evidence of species recruitment and development of hot desert hypolithic communities
Article first published online: 28 OCT 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Environmental Microbiology Reports
Volume 5, Issue 2, pages 219–224, April 2013
How to Cite
Makhalanyane, T. P., Valverde, A., Lacap, D. C., Pointing, S. B., Tuffin, M. I. and Cowan, D. A. (2013), Evidence of species recruitment and development of hot desert hypolithic communities. Environmental Microbiology Reports, 5: 219–224. doi: 10.1111/1758-2229.12003
- Issue published online: 5 MAR 2013
- Article first published online: 28 OCT 2012
- Accepted manuscript online: 4 OCT 2012 07:58AM EST
- Manuscript Accepted: 22 SEP 2012
- Manuscript Revised: 10 SEP 2012
- Manuscript Received: 29 MAY 2012
- Innovation Fund. Grant Number: 71682
- National Research Fund of South Africa
- University of the Western Cape
- Hong Kong Research Grants Council. Grant Number: HKU7733/08HKU7763/10
Fig. S1. Venn diagram comparing the distribution of bacterial T-RFLP fragments between hypolith and soil samples.
Fig. S2. NMDS generated from random ‘resampling’. This was applied in order to demonstrate that the effect of sample size does not influence the overall structure of hypolithic and soil bacterial communities. Code indicators: circles (hypolithons), filled triangles (sub-lithic soil) and empty triangles (open soil). Twelve randomizations are depicted.
Fig. S3. Hypolithic (A) and soil (B) clone library coverage (Good's and CACE) and estimator (Schao1 and SACE) plots.
Fig. S4. Clone libraries distribution for both hypoliths and soil communities.
Fig. S5. Maximum likelihood tree of eubacteria (A) and cyanobacteria (B). Phylotypes recovered during this study are shown in bold type. NCBI GenBank accession tree topologies are supported by Bayesian posterior probabilities (first number) and bootstrap values for 1000 replications (second number). Code indicators: triangles (hypolithons), squares (soil).
Fig. S6. Temperature (A) and % relative humidity (RH) (B) over a 6 month period at the sampling location. Data were acquired at 5 min intervals.
Table S1. BLASTN results against the NCBI database.