The versatile in situ gene expression of an Epsilonproteobacteria-dominated biofilm from a hydrothermal chimney
Article first published online: 20 DEC 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Environmental Microbiology Reports
Volume 5, Issue 2, pages 282–290, April 2013
How to Cite
Dahle, H., Roalkvam, I., Thorseth, I. H., Pedersen, R. B. and Steen, I. H. (2013), The versatile in situ gene expression of an Epsilonproteobacteria-dominated biofilm from a hydrothermal chimney. Environmental Microbiology Reports, 5: 282–290. doi: 10.1111/1758-2229.12016
- Issue published online: 5 MAR 2013
- Article first published online: 20 DEC 2012
- Accepted manuscript online: 15 NOV 2012 10:33AM EST
- Manuscript Accepted: 5 NOV 2012
- Manuscript Revised: 3 OCT 2012
- Manuscript Received: 11 APR 2012
Fig. S1. Picture of the BSROV3 biofilm.
Fig. S2. Picture of the BSROV4 biofilm.
Fig. S3. Rarefaction curves for each sample. The curves are based on OTUs constructed from the 16S rRNA gene amplicons. Error bars show one standard error in each direction.
Fig. S4. MG-RAST analyses of abundance and taxonomic affiliations within SEED subsystem categories. The x-axis and the black bars indicate relative abundances (in per cent) of each functional category. Relative taxonomic composition within each functional category is indicated to the right of each black bar.
Fig. S5. Proposed in situ energy metabolism and CO2 fixation by members of Sulfurovum growing on the surface of hydrothermal chimneys in the Loki's Castle hydrothermal field. SQR, sulfide quinone reductase; FDH, formate dehydrogenase; HYD, hydrogenase; Cyt, cytochrome; NDH, NADH-quinone oxidoreductase; Q, quinone; NAP, nitrate reductase; NIR, nitrite reductase; NOR, nitric oxide reductase; NOS, nitrous oxide reductase; Cbb3, Cbb3-type cytochrome oxidase; rTCA, reverse TCA cycle; ACL, ATP citrate lyase.
Fig. S6. Taxonomic affiliations of transposase transcripts based on BLASTX top hits. The size of each area indicates relative abundance of transcripts for each taxon.
Table S1. Sequence information and diversity indices from analyses of 16S rRNA gene amplicon reads.
Table S2. Sequence information of cDNA reads obtained from BSROV3.
Table S3. Abundances of taxa on phylum and class level from taxonomic assignments of cDNA and amplicon reads.
Appendix S1. Experimental procedures.
Appendix S2. Fasta file of sequences representing an OTU from the amplicon datasets.
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