The Pseudomonas putida HskA hybrid sensor kinase controls the composition of the electron transport chain
Article first published online: 6 DEC 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Environmental Microbiology Reports
Volume 5, Issue 2, pages 291–300, April 2013
How to Cite
Sevilla, E., Silva-Jiménez, H., Duque, E., Krell, T. and Rojo, F. (2013), The Pseudomonas putida HskA hybrid sensor kinase controls the composition of the electron transport chain. Environmental Microbiology Reports, 5: 291–300. doi: 10.1111/1758-2229.12017
- Issue published online: 5 MAR 2013
- Article first published online: 6 DEC 2012
- Accepted manuscript online: 21 NOV 2012 12:09AM EST
- Manuscript Accepted: 13 NOV 2012
- Manuscript Received: 27 JUN 2012
- Spanish Ministry of Science and Innovation. Grant Numbers: BFU2009-07009/BMC, BIO2010-17227, CSD2007-00005
Fig. S1. Model of the P. putida electron transport chain inferred from the genes present in strain KT2440 genome. Electrons flow from different electron donors to the quinone carriers of the cell membrane (UQ) and are then transferred either to the bc1 complex or to the Cyo or CIO terminal ubiquinol oxidases. The bc1 complex feeds the Aa3, Cbb3-1 and Cbb3-2 terminal oxidases. Electrons are finally donated to oxygen. Diverse sensor histidine kinases (HKs) can respond to the redox status of the quinone carriers, undergoing autophosphorylation and triggering an output response.
Table S1. Complete list of genes differentially expressed upon inactivation of the P. putida KT2440 hksA gene (PP_3546), in cells growing exponentially in LB medium.
Table S2. Oligonucleotides used in real-time PCR assays.
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