Experimental procedures.

Fig. S1. Model of the P. putida electron transport chain inferred from the genes present in strain KT2440 genome. Electrons flow from different electron donors to the quinone carriers of the cell membrane (UQ) and are then transferred either to the bc1 complex or to the Cyo or CIO terminal ubiquinol oxidases. The bc1 complex feeds the Aa3, Cbb3-1 and Cbb3-2 terminal oxidases. Electrons are finally donated to oxygen. Diverse sensor histidine kinases (HKs) can respond to the redox status of the quinone carriers, undergoing autophosphorylation and triggering an output response.

Table S1. Complete list of genes differentially expressed upon inactivation of the P. putida KT2440 hksA gene (PP_3546), in cells growing exponentially in LB medium.

Table S2. Oligonucleotides used in real-time PCR assays.

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