Identification of biomass utilizing bacteria in a carbon-depleted glacier forefield soil by the use of 13C DNA stable isotope probing
Article first published online: 16 JAN 2013
© 2013 John Wiley & Sons Ltd and Society for Applied Microbiology
Environmental Microbiology Reports
Thematic Issue: Plant-Microbe Interactions
Volume 5, Issue 3, pages 424–437, June 2013
How to Cite
Zumsteg, A., Schmutz, S. and Frey, B. (2013), Identification of biomass utilizing bacteria in a carbon-depleted glacier forefield soil by the use of 13C DNA stable isotope probing. Environmental Microbiology Reports, 5: 424–437. doi: 10.1111/1758-2229.12027
- Issue published online: 22 APR 2013
- Article first published online: 16 JAN 2013
- Accepted manuscript online: 20 DEC 2012 10:19AM EST
- Manuscript Accepted: 13 DEC 2012
- Manuscript Received: 1 OCT 2012
- Competence Centre Environment and Sustainability (CCES) of the ETH Domain
- Swiss National Science Foundation (project 31003A-138321)
Fig. S1. qPCR of the 16S rRNA gene copy numbers after stable isotope probing of samples amended with either 13C-labelled or non-labelled 12C-biomass. The fractions with a buoyant density of 1.68 g ml−1 were considered to contain non-labelled DNA, whereas the fractions with a buoyant density of 1.72 g ml−1 were considered to contain 13C-labelled DNA.
Table S1. Physico-chemical parameters of the bare soil from the recently deglaciated forefield of the Damma glacier. Soil porosity was measured as pore volume in the corresponding soil volume (m3 m−3). The mineral nutrient contents are given as μmol g−1 soil. Nitrogen (N) and carbon (C) are given in %.
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