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emi412031-sup-0001-fS1.tif3652K

Fig. S1. Diel 16S rRNA gene – DGGE profiles of epiphytic cyanobacteria associated with Cymodocea rotundata. Samples were collected at eight time points during 24 h cycles. The bands marked with black dots and numbers were excised, sequenced and used for the phylogenetic reconstructions in Fig. 2 (As the same time points were used for the diversity analyses these could be regarded as biological replicates.).

emi412031-sup-0002-fS2.tif4521K

Fig. S2. Diel nifH gene (based DNA and RNA) – DGGE profiles of epiphytic cyanobacteria associated with Cymodocea rotundata. A–D. Diel DNA-based gel patterns in 2007 and 2008; and (E–F) diel RNA-based gel patterns in 2008. Samples were collected at eight time points during 24 h cycles. The bands marked with black dots and numbers were excised, sequenced and used for the phylogenetic reconstructions in Fig. 3.

emi412031-sup-0003-fS3.tif287K

Fig. S3. Diel NifH protein profile of epiphytes associated with the phyllosphere of Cymodocea rotundata. Total protein extracts from epiphytes collected in October 2008 were examined using SDS-PAGE and immunoblotting. The 40 kDa size ladder marker is given in Lane 1 (arrow). The molecular weight of the NifH protein was estimated to ~ 38 kDa.

emi412031-sup-0004-suppl.doc91K

Sequences accession number in word file.

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