Fig. S1. Influent and effluent substrate concentration of the enrichment culture.

Fig. S2. Phylogenetic analysis of hzsA gene sequences from the soil anammox enrichment culture. The sequences obtained during this study are indicated with an asterisk. The 18-1 and 18-2 represent the surface layer and the mid layer of enrichment culture respectively. The neighbour-joining tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances to infer the phylogenetic tree. The evolutionary distances were computed using the Jukes–Cantor method and are in the units of the number of base substitutions per site. The bar represents 5% sequence divergence. All ambiguous positions were removed for each sequence pair. There were a total of 2433 positions in the final dataset. Bootstrap values > 50 (1000 replicates) are shown at the branches.

Table S1. PCR primers used in this study.

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