Enrichment of an anammox bacterial community from a flooded paddy soil
Article first published online: 28 FEB 2013
© 2013 John Wiley & Sons Ltd and Society for Applied Microbiology
Environmental Microbiology Reports
Thematic Issue: Plant-Microbe Interactions
Volume 5, Issue 3, pages 483–489, June 2013
How to Cite
Hu, B.-l., Shen, L.-d., Liu, S., Cai, C., Chen, T.-t., Kartal, B., Harhangi, H. R., Op den Camp, H. J. M., Lou, L.-p., Xu, X.-y., Zheng, P. and Jetten, M. S. M. (2013), Enrichment of an anammox bacterial community from a flooded paddy soil. Environmental Microbiology Reports, 5: 483–489. doi: 10.1111/1758-2229.12038
- Issue published online: 22 APR 2013
- Article first published online: 28 FEB 2013
- Accepted manuscript online: 24 JAN 2013 07:10AM EST
- Manuscript Accepted: 18 JAN 2013
- Manuscript Revised: 3 JAN 2013
- Manuscript Received: 9 MAR 2012
- National Natural Science Foundation of China. Grant Numbers: No. 40801198, 51108408
- Natural Science Foundation of Zhejiang Province. Grant Number: Y507227
- Shanghai Tongji Gao Tingyao Environmental Science and Technology Development Foundation
- European Research Council. Grant Number: grant number 2322937
- Netherlands Organization for Scientific Research. Grant Number: grant number 863.11.003
Fig. S1. Influent and effluent substrate concentration of the enrichment culture.
Fig. S2. Phylogenetic analysis of hzsA gene sequences from the soil anammox enrichment culture. The sequences obtained during this study are indicated with an asterisk. The 18-1 and 18-2 represent the surface layer and the mid layer of enrichment culture respectively. The neighbour-joining tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances to infer the phylogenetic tree. The evolutionary distances were computed using the Jukes–Cantor method and are in the units of the number of base substitutions per site. The bar represents 5% sequence divergence. All ambiguous positions were removed for each sequence pair. There were a total of 2433 positions in the final dataset. Bootstrap values > 50 (1000 replicates) are shown at the branches.
Table S1. PCR primers used in this study.
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