Depth-discrete profiles of bacterial communities reveal pronounced spatio-temporal dynamics related to lake stratification

Authors

  • Sarahi L. Garcia,

    1. Jena School for Microbial Communication (JSMC) and Microbial Ecology Group at Friedrich Schiller University, Jena, Germany
    Search for more papers by this author
  • Ivette Salka,

    1. Department 3: Limnology of Stratified Lakes, Leibniz-Institute for Freshwater Ecology and Inland Fisheries, Stechlin, Germany
    Search for more papers by this author
  • Hans-Peter Grossart,

    1. Department 3: Limnology of Stratified Lakes, Leibniz-Institute for Freshwater Ecology and Inland Fisheries, Stechlin, Germany
    2. Institue for Biochemistry and Biology, Postdam University, Potsdam, Germany
    Search for more papers by this author
  • Falk Warnecke

    Corresponding author
    • Jena School for Microbial Communication (JSMC) and Microbial Ecology Group at Friedrich Schiller University, Jena, Germany
    Search for more papers by this author

For correspondence. E-mail falk.warnecke@uni-jena.de; Tel. (+49) 3641 949337; Fax (+49) 3641 949302.

Summary

With this work we intend to stress the importance of considering discrete depth sampling for bacterial community analysis of stratified aquatic systems. Depth is a very important parameter to consider when sampling bacterial communities, as their abundance and composition can change within the distinct water layers. Stratified lakes are good model systems to study these connections since changes in environmental parameters can occur on a cm-scale at the thermo- and/or chemocline. Lake Grosse Fuchskuhle was sampled at discrete fine-scale depths at three time points covering a stage in which the lake was stratified and the beginning of winter mixing. In this paper we present the most abundant bacterial groups at the different depths sampled and also the most abundant operational taxonomic units (OTUs). Overall, oxygen was found to be an important factor shaping the microbial community composition.

Ancillary