Sulfur-oxidizing bacterial populations within cyanobacterial dominated coral disease lesions
Article first published online: 22 APR 2013
© 2013 John Wiley & Sons Ltd and Society for Applied Microbiology
Environmental Microbiology Reports
Thematic Issue on Invertebrate: Microbe Interactions
Volume 5, Issue 4, pages 518–524, August 2013
How to Cite
Bourne, D. G., van der Zee, M. J. J., Botté, E. S. and Sato, Y. (2013), Sulfur-oxidizing bacterial populations within cyanobacterial dominated coral disease lesions. Environmental Microbiology Reports, 5: 518–524. doi: 10.1111/1758-2229.12055
- Issue published online: 15 JUL 2013
- Article first published online: 22 APR 2013
- Accepted manuscript online: 28 MAR 2013 03:42AM EST
- Manuscript Accepted: 20 MAR 2013
- Manuscript Received: 13 DEC 2012
- Australian Institute of Marine Science
- Mitsubishi Corporation
- Earthwatch Institute Australia
- Queensland Government funded Centre for Marine Microbiology and Genetics at AIMS
Supplementary materials and methods.
Fig. S1. Rank abundance charts of OPUs retrieved from CP and BBD lesion samples. OPU-1 dominated retrieved sequences and therefore for clarity, the rank abundance of OPUs 2–20 are inserted in an additional rank abundance display.
Fig. S2. Rarefaction curves based on OPUs retrieved from CP and BBD lesion samples and defined at 90% protein sequence similarity.
Fig. S3. Quantitative real-time PCR assessment of the number of the dominant soxB genes within individual CP and BBD coral disease lesions. The number of soxB genes (OPU-1 type) was calculated as gene copies per ng extracted DNA and normalized relative to the total bacterial population determined by quantitative real-time PCR targeting 16S rRNA genes.
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