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Table S1. Concentrations of SCFA (mmol l−1) and relative standard deviation in the three vessels of the SHIME 1 during the control period (n ≥ 3, per week) and treatment period with AG (n ≥ 3, per week). Significant differences between the weeks have been evaluated by means of one-way anova, and individual means were compared using the Tukey's test. Samples with the same letter are NOT statistically different (P > 0.05).

Table S2. Concentrations of SCFA (mmol l−1) and relative standard deviation in the three vessels of the SHIME 2 during the control period (n ≥ 3, per week) and treatment period with FOS (n ≥ 3, per week). Significant differences between the weeks have been evaluated by means of one-way anova, and individual means were compared using the Tukey's test. Samples with the same letter are NOT statistically different (P > 0.05).

Fig. S1. Results of the SEC analysis conducted on SHIME 1. The MW of the peaks is indicated in the legend of each figure and the relative abundance of the peaks is shown as a pie graph. Data are presented per week of control and treatment in the AC, TC and DC.

Fig. S2. Results of the SEC analysis conducted on SHIME 2. The MW of the peaks is indicated in the legend of each figure and the relative abundance of the peaks is shown as a pie graph. Data are presented per week of control and treatment in the AC, TC and DC.

Fig. S3. Δ total gas production following the treatment with FOS and Fibregum. Significant differences (as compared with the previous sampling point) have been assessed by means of a Student's two-tailed t-test and are indicated with * for P < 0.05.

Fig. S4. Cluster analysis conducted on DGGE analyses of lactobacilli using the unweighted pair group with mathematical averages (UPGMA) and distance matrices of each DGGE based on the Pearson correlation similarity coefficients. S1 refers to the SHIME 1 treated with AG; S2 to the SHIME 2 treated with FOS.

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