Enrichment and isolation of acidophilic sulfate-reducing bacteria from Tinto River sediments
Article first published online: 6 MAY 2013
© 2013 John Wiley & Sons Ltd and Society for Applied Microbiology
Environmental Microbiology Reports
Volume 5, Issue 5, pages 672–678, October 2013
How to Cite
Sánchez-Andrea, I., Stams, A. J. M., Amils, R. and Sanz, J. L. (2013), Enrichment and isolation of acidophilic sulfate-reducing bacteria from Tinto River sediments. Environmental Microbiology Reports, 5: 672–678. doi: 10.1111/1758-2229.12066
- Issue published online: 2 OCT 2013
- Article first published online: 6 MAY 2013
- Accepted manuscript online: 17 APR 2013 05:00AM EST
- Manuscript Accepted: 10 APR 2013
- Manuscript Received: 10 JUL 2012
- ‘Ministerio de Ciencia e Innovación’. Grant Numbers: CTM2009-10521, CGL2009-11059
- Chemical Sciences. Grant Number: 700.55.343
- Netherlands Science Foundation (NWO). Grant Numbers: 323009, F07.001.05
Fig. S1. Screening scheme of the enrichments reflecting the origin of the sediments (JL and SN sampling sites) incubated from pH 4 till pH 6.5 with succinate, glycerol, methanol, lactate or hydrogen as electron donors.
A. Arrows summarize sulfate depletion and organic acids variations.
B. Circles show the selected bottles for further purification.
Table S1. Sulfate concentration (mM) at different sampling times for isolate D-i growing in buffered and non-buffered media. Bars visually indicate the amount of sulfate reduced.
Table S2. Evolution of parameters during growth of isolate D-i on glycerol.
Table S3. Evolution of parameters during growth of isolate D-i in media with L-cysteine (0.5 g l−1) and media with about 5 mM of sulfide as starting concentration. Bars visually indicate the amount of sulfate reduced.
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